Peak Name: ISTD Use as Internal Standard Q1/Q3 masses: 314.20/286.10 amu Peak Name: CmpdX Internal Standard: ISTD Q1/Q3 masses: 309.20/281.10 amu Fit Linear weighting OneOverX Iterate N/A Intercept -0.0026 slope 0.0157 Correlation coefficient 0.990043892 Use Area Sample Name Sample ID Filetype Sample Desc. Set Number Acquisition Method Date Of Acq. Rack Type Rack Number Vial Position Plate Type Plate Number Filename Dil.Factor Weight To Volume Ratio Sample Annotation Peak Name Units Area Analyte Peak Height for DAD Height Analyte Annotation Conc. R.T. Expected RT RT Window Centroid Location Start Scan Start Time End Scan End Time Integ.Type S/N Peak Width Standard Query Status Analyte Mass Range Analyte Peak Area for DAD Analyte/IS Area Analyte/IS Height Analyte Wavelength Ranges Analyte UV Range Analyte Peak Width at 50% Height Analyte Slope of Baseline Analyte Processing Alg. Analyte Peak Asymmetry IS Peak Name IS Units IS Area IS Peak Area for DAD IS Height IS Peak Height for DAD IS Conc. IS R.T. IS Expected RT IS RT Window IS Centroid Location IS Start Scan IS Start Time IS Stop Scan IS End Time IS Integ.Type IS S/N IS Peak Width IS Mass Range IS Wavelength Ranges IS UV Range IS Peak Width at 50% Height IS Slope of Baseline IS Processing Alg. IS Peak Asymmetry Use Record Record Modified Calc.Conc. Calculated Concentration for DAD Rel.R.T. Accuracy Resp.Factor 1 001 EBA_Interface_2009_01 blank 1 1 Unknown 0 1290-AJS-Alprazolam-0.5uL-2MRM-50C-082809.m 8/28/2009 10:10 P1-A1 PlateOrVial 1 001 EBA_Interface_2009_01 blank 1.d 1 0 CmpdX ng/ml 11 N/A 32 N/A 149.764 0.922 0.576 10 0 0 0.919 0 0.938 Inspect 0.4 0.0192 N/A 309.2 -> 281.1 N/A 2.3491 0.9455 0 N/A 0 0 AutoMagic_LC 0 ISTD ng/ml 5 0 34 0 1.0 0.694 0.573 10 0 0 0.6916 0 0.7019 Inspect 0.4 0.010 314.2 -> 286.1 0 N/A 0 0 AutoMagic_LC 0 0 0 149.764 0 1.329 0.0 0.016067 7 8 1 002 EBA_Interface_2009_01 CONTROL_BLANK 1 2 Unknown 0 1290-AJS-Alprazolam-0.5uL-2MRM-50C-082809.m 8/28/2009 10:12 P1-A1 PlateOrVial 1 002 EBA_Interface_2009_01 CONTROL_BLANK 1.d 1 0 CmpdX ng/ml 11 N/A 56 N/A 153.663 0.888 0.576 10 0 0 0.875 0 0.896 Inspect 0.7 0.0208 N/A 309.2 -> 281.1 N/A 2.4103 2.3813 0 N/A 0 0 AutoMagic_LC 0 ISTD ng/ml 5 0 24 0 1.0 0.882 0.573 10 0 0 0.8757 0 0.8835 Inspect 0.5 0.008 314.2 -> 286.1 0 N/A 0 0 AutoMagic_LC 0 0 0 153.663 0 1.007 0.0 0.016067 7 8 1 003 EBA_Interface_2009_01 STD01 1 1 3 Standard 0 1290-AJS-Alprazolam-0.5uL-2MRM-50C-082809.m 8/28/2009 10:15 P1-A2 PlateOrVial 1 003 EBA_Interface_2009_01 STD01 1 1.d 1 0 CmpdX ng/ml 93 N/A 237 N/A 0.918 0.576 0.576 10 0 0 0.566 0 0.590 Accepted 4.8 0.0239 N/A 309.2 -> 281.1 N/A 0.0118 0.0187 0 N/A 0 0 AutoMagic_LC 0 ISTD ng/ml 7845 0 12696 0 1.0 0.572 0.573 10 0 0 0.3736 0 0.6039 Rejected 143.8 0.230 314.2 -> 286.1 0 N/A 0 0 AutoMagic_LC 0 0 0 0.918 0 1.006 91.8 0.016067 7 8 1 004 EBA_Interface_2009_01 STD02 1 1 4 Standard 0 1290-AJS-Alprazolam-0.5uL-2MRM-50C-082809.m 8/28/2009 10:17 P1-A3 PlateOrVial 1 004 EBA_Interface_2009_01 STD02 1 1.d 1 0 CmpdX ng/ml 825 N/A 1746 N/A 7.481 0.576 0.576 10 0 0 0.564 0 0.595 Accepted 15.4 0.0310 N/A 309.2 -> 281.1 N/A 0.1149 0.1600 0 N/A 0 0 AutoMagic_LC 0 ISTD ng/ml 7182 0 10911 0 1.0 0.572 0.573 10 0 0 0.3632 0 0.6002 Rejected 139.5 0.237 314.2 -> 286.1 0 N/A 0 0 AutoMagic_LC 0 0 0 7.481 0 1.007 74.8 0.016067 7 8 1 005 EBA_Interface_2009_01 STD03 1 1 5 Standard 0 1290-AJS-Alprazolam-0.5uL-2MRM-50C-082809.m 8/28/2009 10:19 P1-A4 PlateOrVial 1 005 EBA_Interface_2009_01 STD03 1 1.d 1 0 CmpdX ng/ml 11769 N/A 19212 N/A 134.785 0.576 0.576 10 0 0 0.278 0 0.607 Rejected 202.1 0.3289 N/A 309.2 -> 281.1 N/A 2.1139 2.2582 0 N/A 0 0 AutoMagic_LC 0 ISTD ng/ml 5567 0 8507 0 1.0 0.573 0.573 10 0 0 0.3528 0 0.6025 Rejected 130.4 0.250 314.2 -> 286.1 0 N/A 0 0 AutoMagic_LC 0 0 0 134.785 0 1.005 134.8 0.016067 7 8 1 006 EBA_Interface_2009_01 0001 PO 0m PLM-1 1 6 Unknown 0 1290-AJS-Alprazolam-0.5uL-2MRM-50C-082809.m 8/28/2009 10:22 P1-A1 PlateOrVial 1 006 EBA_Interface_2009_01 0001 PO 0m PLM-1 1.d 1 0 CmpdX ng/ml 7 N/A 26 N/A 136.227 0.960 0.576 10 0 0 0.956 0 0.965 Inspect 0.5 0.0090 N/A 309.2 -> 281.1 N/A 2.1365 1.2516 0 N/A 0 0 AutoMagic_LC 0 ISTD ng/ml 3 0 21 0 1.0 0.591 0.573 10 0 0 0.5839 0 0.5916 Inspect 0.4 0.008 314.2 -> 286.1 0 N/A 0 0 AutoMagic_LC 0 0 0 136.227 0 1.624 0.0 0.016067 7 8 1 007 EBA_Interface_2009_01 0001 PO 5m PLM-1 1 7 Unknown 0 1290-AJS-Alprazolam-0.5uL-2MRM-50C-082809.m 8/28/2009 10:24 P1-A5 PlateOrVial 1 007 EBA_Interface_2009_01 0001 PO 5m PLM-1 1.d 1 0 CmpdX ng/ml 2596 N/A 4216 N/A 42.437 0.575 0.576 10 0 0 0.418 0 0.595 Rejected 80.4 0.1761 N/A 309.2 -> 281.1 N/A 0.6638 0.6972 0 N/A 0 0 AutoMagic_LC 0 ISTD ng/ml 3911 0 6047 0 1.0 0.573 0.573 10 0 0 0.4170 0 0.5933 Rejected 83.3 0.176 314.2 -> 286.1 0 N/A 0 0 AutoMagic_LC 0 0 0 42.437 0 1.004 0.0 0.016067 7 8 1 008 EBA_Interface_2009_01 0001 PO 15m PLM-1 1 8 Unknown 0 1290-AJS-Alprazolam-0.5uL-2MRM-50C-082809.m 8/28/2009 10:27 P1-A9 PlateOrVial 1 008 EBA_Interface_2009_01 0001 PO 15m PLM-1 1.d 1 0 CmpdX ng/ml 5334 N/A 8616 N/A 69.453 0.576 0.576 10 0 0 0.407 0 0.597 Rejected 114.1 0.1903 N/A 309.2 -> 281.1 N/A 1.0880 1.1104 0 N/A 0 0 AutoMagic_LC 0 ISTD ng/ml 4903 0 7760 0 1.0 0.573 0.573 10 0 0 0.3703 0 0.5976 Rejected 129.4 0.227 314.2 -> 286.1 0 N/A 0 0 AutoMagic_LC 0 0 0 69.453 0 1.005 0.0 0.016067 7 8 1 009 EBA_Interface_2009_01 0001 PO 30m PLM-1 1 9 Unknown 0 1290-AJS-Alprazolam-0.5uL-2MRM-50C-082809.m 8/28/2009 10:29 P1-A4 PlateOrVial 1 009 EBA_Interface_2009_01 0001 PO 30m PLM-1 1.d 1 0 CmpdX ng/ml 11427 N/A 17175 N/A 131.069 0.576 0.576 10 0 0 0.294 0 0.608 Rejected 173.9 0.3141 N/A 309.2 -> 281.1 N/A 2.0555 2.0812 0 N/A 0 0 AutoMagic_LC 0 ISTD ng/ml 5559 0 8253 0 1.0 0.573 0.573 10 0 0 0.4309 0 0.5976 Rejected 90.6 0.167 314.2 -> 286.1 0 N/A 0 0 AutoMagic_LC 0 0 0 131.069 0 1.006 0.0 0.016067 7 8 1 010 EBA_Interface_2009_01 0001 PO 1h PLM-1 1 10 Unknown 0 1290-AJS-Alprazolam-0.5uL-2MRM-50C-082809.m 8/28/2009 10:31 P1-A6 PlateOrVial 1 010 EBA_Interface_2009_01 0001 PO 1h PLM-1 1.d 1 0 CmpdX ng/ml 29894 N/A 45719 N/A 346.616 0.576 0.576 10 0 0 0.238 0 0.636 Rejected 846.1 0.3983 N/A 309.2 -> 281.1 N/A 5.4402 5.0117 0 N/A 0 0 AutoMagic_LC 0 ISTD ng/ml 5495 0 9122 0 1.0 0.572 0.573 10 0 0 0.3919 0 0.5976 Rejected 131.6 0.206 314.2 -> 286.1 0 N/A 0 0 AutoMagic_LC 0 0 0 346.616 0 1.006 0.0 0.016067 7 8 1 011 EBA_Interface_2009_01 0001 PO 2h PLM-1 1 11 Unknown 0 1290-AJS-Alprazolam-0.5uL-2MRM-50C-082809.m 8/28/2009 10:34 P1-A7 PlateOrVial 1 011 EBA_Interface_2009_01 0001 PO 2h PLM-1 1.d 1 0 CmpdX ng/ml 62323 N/A 95822 N/A 727.569 0.577 0.576 10 0 0 0.239 0 0.620 Rejected 1550.2 0.3811 N/A 309.2 -> 281.1 N/A 11.4221 10.8497 0 N/A 0 0 AutoMagic_LC 0 ISTD ng/ml 5456 0 8832 0 1.0 0.572 0.573 10 0 0 0.3683 0 0.5977 Rejected 133.6 0.229 314.2 -> 286.1 0 N/A 0 0 AutoMagic_LC 0 0 0 727.569 0 1.007 0.0 0.016067 7 8 1 012 EBA_Interface_2009_01 0001 PO 7h PLM-1 1 12 Unknown 0 1290-AJS-Alprazolam-0.5uL-2MRM-50C-082809.m 8/28/2009 10:36 P1-A8 PlateOrVial 1 012 EBA_Interface_2009_01 0001 PO 7h PLM-1 1.d 1 0 CmpdX ng/ml 127859 N/A 192751 N/A 953.185 0.576 0.576 10 0 0 0.239 0 0.623 Rejected 2043.1 0.3840 N/A 309.2 -> 281.1 N/A 14.9648 14.0644 0 N/A 0 0 AutoMagic_LC 0 ISTD ng/ml 8544 0 13705 0 1.0 0.573 0.573 10 0 0 0.3467 0 0.6044 Rejected 190.9 0.258 314.2 -> 286.1 0 N/A 0 0 AutoMagic_LC 0 0 0 953.185 0 1.006 0.0 0.016067 7 8 1 013 EBA_Interface_2009_01 0001 PO 24h PLM-1 1 13 Unknown 0 1290-AJS-Alprazolam-0.5uL-2MRM-50C-082809.m 8/28/2009 10:38 P1-A6 PlateOrVial 1 013 EBA_Interface_2009_01 0001 PO 24h PLM-1 1.d 1 0 CmpdX ng/ml 30199 N/A 46033 N/A 246.892 0.576 0.576 10 0 0 0.302 0 0.619 Rejected 206.4 0.3171 N/A 309.2 -> 281.1 N/A 3.8742 4.0087 0 N/A 0 0 AutoMagic_LC 0 ISTD ng/ml 7795 0 11483 0 1.0 0.573 0.573 10 0 0 0.3588 0 0.6027 Rejected 137.3 0.244 314.2 -> 286.1 0 N/A 0 0 AutoMagic_LC 0 0 0 246.892 0 1.005 0.0 0.016067 7 8 1 014 EBA_Interface_2009_01 QC01 1 1 14 QC 0 1290-AJS-Alprazolam-0.5uL-2MRM-50C-082809.m 8/28/2009 10:41 P1-A5 PlateOrVial 1 014 EBA_Interface_2009_01 QC01 1 1.d 1 0 CmpdX ng/ml 2554 N/A 3798 N/A 34.734 0.577 0.576 10 0 0 0.424 0 0.601 Rejected 57.0 0.1770 N/A 309.2 -> 281.1 N/A 0.5428 0.4916 0 N/A 0 0 AutoMagic_LC 0 ISTD ng/ml 4706 0 7725 0 1.0 0.573 0.573 10 0 0 0.3797 0 0.5933 Rejected 125.5 0.214 314.2 -> 286.1 0 N/A 0 0 AutoMagic_LC 0 0 0 34.734 0 1.008 138.9 0.016067 7 8 1 015 EBA_Interface_2009_01 QC02 1 1 15 QC 0 1290-AJS-Alprazolam-0.5uL-2MRM-50C-082809.m 8/28/2009 10:43 P1-A6 PlateOrVial 1 015 EBA_Interface_2009_01 QC02 1 1.d 1 0 CmpdX ng/ml 29961 N/A 47382 N/A 241.115 0.576 0.576 10 0 0 0.292 0 0.617 Rejected 223.5 0.3246 N/A 309.2 -> 281.1 N/A 3.7835 3.6805 0 N/A 0 0 AutoMagic_LC 0 ISTD ng/ml 7919 0 12874 0 1.0 0.572 0.573 10 0 0 0.3562 0 0.6003 Rejected 178.8 0.244 314.2 -> 286.1 0 N/A 0 0 AutoMagic_LC 0 0 0 241.115 0 1.006 96.4 0.016067 7 8 1 016 EBA_Interface_2009_01 0002 PO 0m PLM-1 1 16 Unknown 0 1290-AJS-Alprazolam-0.5uL-2MRM-50C-082809.m 8/28/2009 10:46 P1-A1 PlateOrVial 1 016 EBA_Interface_2009_01 0002 PO 0m PLM-1 1.d 1 0 CmpdX ng/ml 14 N/A 38 N/A 250.006 0.582 0.576 10 0 0 0.568 0 0.589 Inspect 0.4 0.0213 N/A 309.2 -> 281.1 N/A 3.9231 1.5459 0 N/A 0 0 AutoMagic_LC 0 ISTD ng/ml 4 0 25 0 1.0 0.858 0.573 10 0 0 0.8557 0 0.8608 Rejected 0.6 0.005 314.2 -> 286.1 0 N/A 0 0 AutoMagic_LC 0 0 0 250.006 0 0.678 0.0 0.016067 7 8 1 017 EBA_Interface_2009_01 0002 PO 5m PLM-1 1 17 Unknown 0 1290-AJS-Alprazolam-0.5uL-2MRM-50C-082809.m 8/28/2009 10:48 P1-A5 PlateOrVial 1 017 EBA_Interface_2009_01 0002 PO 5m PLM-1 1.d 1 0 CmpdX ng/ml 2148 N/A 3868 N/A 25.466 0.576 0.576 10 0 0 0.564 0 0.600 Accepted 33.0 0.0362 N/A 309.2 -> 281.1 N/A 0.3973 0.4579 0 N/A 0 0 AutoMagic_LC 0 ISTD ng/ml 5406 0 8446 0 1.0 0.573 0.573 10 0 0 0.3640 0 0.5950 Rejected 127.4 0.231 314.2 -> 286.1 0 N/A 0 0 AutoMagic_LC 0 0 0 25.466 0 1.005 0.0 0.016067 7 8 1 018 EBA_Interface_2009_01 0002 PO 15m PLM-1 1 18 Unknown 0 1290-AJS-Alprazolam-0.5uL-2MRM-50C-082809.m 8/28/2009 10:50 P1-A9 PlateOrVial 1 018 EBA_Interface_2009_01 0002 PO 15m PLM-1 1.d 1 0 CmpdX ng/ml 5648 N/A 9014 N/A 59.491 0.577 0.576 10 0 0 0.375 0 0.602 Rejected 132.4 0.2275 N/A 309.2 -> 281.1 N/A 0.9316 0.9549 0 N/A 0 0 AutoMagic_LC 0 ISTD ng/ml 6062 0 9440 0 1.0 0.572 0.573 10 0 0 0.4197 0 0.6002 Rejected 106.9 0.181 314.2 -> 286.1 0 N/A 0 0 AutoMagic_LC 0 0 0 59.491 0 1.009 0.0 0.016067 7 8 1 019 EBA_Interface_2009_01 0002 PO 30m PLM-1 1 19 Unknown 0 1290-AJS-Alprazolam-0.5uL-2MRM-50C-082809.m 8/28/2009 10:53 P1-A4 PlateOrVial 1 019 EBA_Interface_2009_01 0002 PO 30m PLM-1 1.d 1 0 CmpdX ng/ml 11533 N/A 18156 N/A 124.954 0.577 0.576 10 0 0 0.344 0 0.602 Rejected 174.7 0.2583 N/A 309.2 -> 281.1 N/A 1.9595 2.0084 0 N/A 0 0 AutoMagic_LC 0 ISTD ng/ml 5886 0 9040 0 1.0 0.573 0.573 10 0 0 0.4100 0 0.5968 Rejected 106.3 0.187 314.2 -> 286.1 0 N/A 0 0 AutoMagic_LC 0 0 0 124.954 0 1.006 0.0 0.016067 7 8 1 020 EBA_Interface_2009_01 0002 PO 1h PLM-1 1 20 Unknown 0 1290-AJS-Alprazolam-0.5uL-2MRM-50C-082809.m 8/28/2009 10:55 P1-A6 PlateOrVial 1 020 EBA_Interface_2009_01 0002 PO 1h PLM-1 1.d 1 0 CmpdX ng/ml 29718 N/A 44981 N/A 233.426 0.577 0.576 10 0 0 0.240 0 0.637 Rejected 828.2 0.3973 N/A 309.2 -> 281.1 N/A 3.6628 3.8343 0 N/A 0 0 AutoMagic_LC 0 ISTD ng/ml 8113 0 11731 0 1.0 0.574 0.573 10 0 0 0.3588 0 0.6017 Rejected 145.2 0.243 314.2 -> 286.1 0 N/A 0 0 AutoMagic_LC 0 0 0 233.426 0 1.006 0.0 0.016067 7 8 1 021 EBA_Interface_2009_01 0002 PO 2h PLM-1 1 21 Unknown 0 1290-AJS-Alprazolam-0.5uL-2MRM-50C-082809.m 8/28/2009 10:58 P1-A7 PlateOrVial 1 021 EBA_Interface_2009_01 0002 PO 2h PLM-1 1.d 1 0 CmpdX ng/ml 62595 N/A 99416 N/A 596.009 0.576 0.576 10 0 0 0.239 0 0.630 Rejected 1567.6 0.3912 N/A 309.2 -> 281.1 N/A 9.3563 9.2111 0 N/A 0 0 AutoMagic_LC 0 ISTD ng/ml 6690 0 10793 0 1.0 0.572 0.573 10 0 0 0.3875 0 0.6002 Rejected 147.3 0.213 314.2 -> 286.1 0 N/A 0 0 AutoMagic_LC 0 0 0 596.009 0 1.008 0.0 0.016067 7 8 1 022 EBA_Interface_2009_01 0002 PO 7h PLM-1 1 22 Unknown 0 1290-AJS-Alprazolam-0.5uL-2MRM-50C-082809.m 8/28/2009 11:00 P1-A8 PlateOrVial 1 022 EBA_Interface_2009_01 0002 PO 7h PLM-1 1.d 1 0 CmpdX ng/ml 134709 N/A 198147 N/A 945.579 0.578 0.576 10 0 0 0.238 0 0.622 Rejected 1981.8 0.3840 N/A 309.2 -> 281.1 N/A 14.8454 14.2508 0 N/A 0 0 AutoMagic_LC 0 ISTD ng/ml 9074 0 13904 0 1.0 0.573 0.573 10 0 0 0.3641 0 0.6088 Rejected 178.9 0.245 314.2 -> 286.1 0 N/A 0 0 AutoMagic_LC 0 0 0 945.579 0 1.009 0.0 0.016067 7 8 1 023 EBA_Interface_2009_01 0002 PO 24h PLM-1 1 23 Unknown 0 1290-AJS-Alprazolam-0.5uL-2MRM-50C-082809.m 8/28/2009 11:02 P1-A6 PlateOrVial 1 023 EBA_Interface_2009_01 0002 PO 24h PLM-1 1.d 1 0 CmpdX ng/ml 31392 N/A 53248 N/A 235.119 0.576 0.576 10 0 0 0.298 0 0.609 Rejected 234.9 0.3101 N/A 309.2 -> 281.1 N/A 3.6894 4.3006 0 N/A 0 0 AutoMagic_LC 0 ISTD ng/ml 8509 0 12381 0 1.0 0.573 0.573 10 0 0 0.3510 0 0.6080 Rejected 153.8 0.257 314.2 -> 286.1 0 N/A 0 0 AutoMagic_LC 0 0 0 235.119 0 1.006 0.0 0.016067 7 8 1 024 EBA_Interface_2009_01 STD04 1 1 24 Standard 0 1290-AJS-Alprazolam-0.5uL-2MRM-50C-082809.m 8/28/2009 11:05 P1-A7 PlateOrVial 1 024 EBA_Interface_2009_01 STD04 1 1.d 1 0 CmpdX ng/ml 63180 N/A 104734 N/A 518.386 0.576 0.576 10 0 0 0.239 0 0.617 Rejected 1504.3 0.3779 N/A 309.2 -> 281.1 N/A 8.1374 9.0087 0 N/A 0 0 AutoMagic_LC 0 ISTD ng/ml 7764 0 11626 0 1.0 0.574 0.573 10 0 0 0.3562 0 0.6011 Rejected 145.1 0.245 314.2 -> 286.1 0 N/A 0 0 AutoMagic_LC 0 0 0 518.386 0 1.003 103.7 0.016067 7 8 1 025 EBA_Interface_2009_01 STD05 1 1 25 Standard 0 1290-AJS-Alprazolam-0.5uL-2MRM-50C-082809.m 8/28/2009 11:07 P1-A8 PlateOrVial 1 025 EBA_Interface_2009_01 STD05 1 1.d 1 0 CmpdX ng/ml 135645 N/A 201832 N/A 949.431 0.577 0.576 10 0 0 0.238 0 0.627 Rejected 2052.5 0.3892 N/A 309.2 -> 281.1 N/A 14.9059 14.6184 0 N/A 0 0 AutoMagic_LC 0 ISTD ng/ml 9100 0 13807 0 1.0 0.572 0.573 10 0 0 0.3345 0 0.6062 Rejected 193.2 0.272 314.2 -> 286.1 0 N/A 0 0 AutoMagic_LC 0 0 0 949.431 0 1.007 94.9 0.016067 7 8